Vietnam develops first protocol for sperm cryopreservation of tiger grouper

Ruby Gonzalez
October 01, 2018
By Ruby Gonzalez
Vietnam develops first protocol for sperm cryopreservation of tiger grouper
Photo: Fotolia by Adobe Stock
A university and government agency in Vietnam worked together to develop the country’s first protocol for sperm cryopreservation of tiger grouper fuscoguttatus with the goal of providing an all-year-round supply for seed production.

Minh Hoan Le of Nha Trang University and Thuy Thanh Thi Nguyen of the Research Institute for Aquaculture focused on finding the best extender, cryoprotectant, dilution ratio, cryopreserved volume and freezing method for sperm cryopreservation of tiger grouper.

The most effective protocol used 15 percent dimethyl sulfoxide (DMSO) as cryoprotectant to semen in a two-step freezing method with temperatures set at -80°C and -196°C.

Talking about the results of this protocol they said, “…the fertilization rate and hatching rate of the post-thawed sperm cryopreserved for one week, one month, or one year in liquid nitrogen were similar to that of fresh sperm.”

The authors, in the abstract of “Sperm cryopreservation of tiger grouper Epinephelus fuscoguttatus,” noted that the source of male tiger grouper broodstock is limited and collecting semen for seed production is not always possible.

Summing up the advantages of the method, they said, “Cryopreservation of tiger grouper semen would reduce the number of males needed, minimize handling stress through less frequent stripping, facilitate artificial propagation when ova are available, promote genetic and breeding studies, and enhance seed production on a commercial scale.”

The high-value species is an important candidate for marine aquaculture of Vietnam but undependable supply of larvae is putting a cap on its potential.

In 2016, Vietnam exported grouper valued at US$42 million. It is ranked 27th in terms of global export market share. During the same period, it imported grouper valued at US$159 million.

Tiger grouper is a perennial favourite in the live fish markets of Southeast and Northeast Asia, where it commands a premium price.

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